E-ISSN 2577-5669
 

Original Research 


Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection

Hawraa F. Hussein1, Ilham A. Bunyan2*.

Abstract
A total of 100 samples were collected from patients suffering from urinary tract infections were admitted
and visited Maternity and Children Hospital in Al-Hilla city, during a period of three months from
September to November 2021. To confirm the identification of E. coli by use selective media,
biochemical tests, Api20E system and automated Vitek 2 system, it was found that, 33 isolates were
showed positive and identified as E. coli. Molecular detection of some virulence factors genes were
studied, out of 33 E. coli isolates, fimH gene was detected in 27(81.8%) isolates by the presence of (508
bp), pap gene was detected in 26(78.8%) isolates by the presence of (336bp), hlyA gene was detected in
10(30.3%) isolates by the presence of (1177bp), feoB gene was detected in 19(57.6%) isolates by the
presence of (470bp), usp gene was detected in 21(63.6%%) isolates by the presence of (440bp) and
24(72.7%) have iroN gene by the presence of (665bp) compared with allelic ladder.

Key words: Genes, PCR, E. coli, Urinary tract infections


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hawraa F. Hussein1
Articles by Ilham A. Bunyan2*
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

HFH, Bunyan IA. Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. J Complement Med Res. 2022; 13(1): 129-137. doi:10.5455/jcmr.2022.13.01.23


Web Style

HFH, Bunyan IA. Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. https://www.jocmr.com/?mno=5257 [Access: July 02, 2022]. doi:10.5455/jcmr.2022.13.01.23


AMA (American Medical Association) Style

HFH, Bunyan IA. Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. J Complement Med Res. 2022; 13(1): 129-137. doi:10.5455/jcmr.2022.13.01.23



Vancouver/ICMJE Style

HFH, Bunyan IA. Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. J Complement Med Res. (2022), [cited July 02, 2022]; 13(1): 129-137. doi:10.5455/jcmr.2022.13.01.23



Harvard Style

, H. F. H. & Bunyan, . I. A. (2022) Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. J Complement Med Res, 13 (1), 129-137. doi:10.5455/jcmr.2022.13.01.23



Turabian Style

, Hawraa F. Hussein, and Ilham A. Bunyan. 2022. Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. Journal of Complementary Medicine Research, 13 (1), 129-137. doi:10.5455/jcmr.2022.13.01.23



Chicago Style

, Hawraa F. Hussein, and Ilham A. Bunyan. "Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection." Journal of Complementary Medicine Research 13 (2022), 129-137. doi:10.5455/jcmr.2022.13.01.23



MLA (The Modern Language Association) Style

, Hawraa F. Hussein, and Ilham A. Bunyan. "Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection." Journal of Complementary Medicine Research 13.1 (2022), 129-137. Print. doi:10.5455/jcmr.2022.13.01.23



APA (American Psychological Association) Style

, H. F. H. & Bunyan, . I. A. (2022) Molecular Study of Some Important Genes among E. coli Isolated from Urinary Tract Infection. Journal of Complementary Medicine Research, 13 (1), 129-137. doi:10.5455/jcmr.2022.13.01.23