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Original Research 


Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract

Alex Boye, Victor Yao Atsu Barku, Ernest Amponsah Asiamah, Adamu Yussam.

Abstract
Background: Previously, folk claims of P. fruticosa were ascertained pharmacologically; and its safety studied, but its effect/safety on female reproductive system remained unknown. Objective: The study assessed P. fruticosa leaf extract (PFE) on follicular development in non-pregnant rats; implantation and post-implantation loss in pregnant rats. Methods: The study used healthy adult non-pregnant and pregnant female and male Wistar rats (150-200 g). Non-pregnant rats were randomly assigned to five groups: normal saline (5 ml/kg po), Clomiphene citrate (50 mg/kg po) and PFE (100, 200, 500 mg/kg po) and treated once daily for 21 days. Dams were sacrificed under deep anesthesia on day 22. ELISA kit was used to measure serum estrogen, FSH and LH. Uterus and ovary were histologically assessed. Dams were co-habited with fertile males for one week; confirmed day 1 pregnant rats were randomly re-assigned to five groups with Misoprostol (200 mg/kg) as reference and treated once daily for 15 days. Implantation and post-implantation loss were assessed (6 and 15 gestations). Results: PFE and clomiphene citrate increased follicular development at the primordial and primary follicle stages compared to control. PFE improved uterine musculature compared to control. PFE decreased serum FSH but increased (P<0.05) serum estrogen and LH compared to control. PFE increased gravid uterine weight compared to control. Total implantation sites were comparable across all groups. Misoprostol and PFE (500 mg/kg) produced post-implantation loss compared to control. Conclusion: PFE (≤100 mg/kg) improved follicular development in non-pregnant rats, but pose risk of post-implantation loss in pregnant rats at ≥ 500 mg/kg.

Key words: Follicular development, Polyscias fruticosa, Post-implantation loss


 
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Pubmed Style

Boye A, Barku VYA, Asiamah EA, Yussam A. Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. J Complement Med Res. 2018; 8(1): 1-10. doi:10.5455/jcmr.20180210021526


Web Style

Boye A, Barku VYA, Asiamah EA, Yussam A. Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. http://www.jocmr.com/?mno=289628 [Access: June 21, 2018]. doi:10.5455/jcmr.20180210021526


AMA (American Medical Association) Style

Boye A, Barku VYA, Asiamah EA, Yussam A. Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. J Complement Med Res. 2018; 8(1): 1-10. doi:10.5455/jcmr.20180210021526



Vancouver/ICMJE Style

Boye A, Barku VYA, Asiamah EA, Yussam A. Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. J Complement Med Res. (2018), [cited June 21, 2018]; 8(1): 1-10. doi:10.5455/jcmr.20180210021526



Harvard Style

Boye, A., Barku, V. Y. A., Asiamah, E. A. & Yussam, A. (2018) Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. J Complement Med Res, 8 (1), 1-10. doi:10.5455/jcmr.20180210021526



Turabian Style

Boye, Alex, Victor Yao Atsu Barku, Ernest Amponsah Asiamah, and Adamu Yussam. 2018. Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. Journal of Complementary Medicine Research, 8 (1), 1-10. doi:10.5455/jcmr.20180210021526



Chicago Style

Boye, Alex, Victor Yao Atsu Barku, Ernest Amponsah Asiamah, and Adamu Yussam. "Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract." Journal of Complementary Medicine Research 8 (2018), 1-10. doi:10.5455/jcmr.20180210021526



MLA (The Modern Language Association) Style

Boye, Alex, Victor Yao Atsu Barku, Ernest Amponsah Asiamah, and Adamu Yussam. "Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract." Journal of Complementary Medicine Research 8.1 (2018), 1-10. Print. doi:10.5455/jcmr.20180210021526



APA (American Psychological Association) Style

Boye, A., Barku, V. Y. A., Asiamah, E. A. & Yussam, A. (2018) Follicular Development and Post-implantation Loss Assessments in non-pregnant and pregnant rats orally Exposed to Polyscias Fruticosa Leaf Extract. Journal of Complementary Medicine Research, 8 (1), 1-10. doi:10.5455/jcmr.20180210021526