Journal of Complementary Medicine Research

2018, Vol: 8, Issue: 1

8/1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Required files to uploaded

Cover Letter
Title Page

« Previous Article

Original Research

J Intercult Ethnopharmacol. 2018; 7(1): 66-75

doi: 10.5455/jice.20171013045424

Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal

Rupak Thapa, Anil Upreti, Bishnu Prasad Pandey.

Abstract
Aims: The present study aims to investigate chemical composition and biological activities of Pinus roxburghii collected from Kavre district of Nepal.
Material and Methods: Phytochemical screening, antibacterial activities and antioxidant activities were measured. Total phenolic content and total flavonoid content was determined using the spectrophotometric analysis. Chemical composition was carried out using GC-MS analysis.
Results: Phytochemical analysis reveals the presence of interesting metabolites like cardiac glycosides, saponin, protein, quinone, sterols, tannin and terpenoids. Highest total phenolic content and total flavonoid content was observed in bark crude methanol extract. Result further revealed that bark methanol extract showed the highest antioxidant activity. Furthermore, methanol and acetone extract of cone, bark and needle showed range of in-vitro antibacterial activity against gram positive and gram negative pathogens. Gas chromatography mass spectroscopy analysis of crude acetone extract of bark revealed the presence of 14 different compounds. The major chemical constituents identified in the bark extract are 1,8 cineole, linalool, beta-thujone, chrysantheone, camphor and terpinen-4-ol.
Conclusions: This study showed that among that needle, cone and bark of Pinus roxburghii as a huge source of biological active metabolites. Furthermore, bark extract revealed the presence of diverse chemical constituent.

Key words: Pinus roxburghii, phytochemical screening, total phenol content, total flavonoid content, antioxidant activities and GC-MS

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	Print this Article
	How to cite this article
	Export to
	Export to
	Related Records
	Articles by Rupak Thapa Articles by Anil Upreti Articles by Bishnu Prasad Pandey
	on Google
	on Google Scholar
	Article Statistics
	Viewed: 710 Downloaded: 305 Cited: 0

How to Cite this Article

Pubmed Style

Thapa R, Upreti A, Pandey BP. Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. J Complement Med Res. 2018; 7(1): 66-75. doi:10.5455/jice.20171013045424

Web Style

Thapa R, Upreti A, Pandey BP. Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. http://www.jocmr.com/?mno=280246 [Access: April 23, 2018]. doi:10.5455/jice.20171013045424

AMA (American Medical Association) Style

Thapa R, Upreti A, Pandey BP. Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. J Complement Med Res. 2018; 7(1): 66-75. doi:10.5455/jice.20171013045424

Vancouver/ICMJE Style

Thapa R, Upreti A, Pandey BP. Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. J Complement Med Res. (2018), [cited April 23, 2018]; 7(1): 66-75. doi:10.5455/jice.20171013045424

Harvard Style

Thapa, R., Upreti, A. & Pandey, B. P. (2018) Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. J Complement Med Res, 7 (1), 66-75. doi:10.5455/jice.20171013045424

Turabian Style

Thapa, Rupak, Anil Upreti, and Bishnu Prasad Pandey. 2018. Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. Journal of Complementary Medicine Research, 7 (1), 66-75. doi:10.5455/jice.20171013045424

Chicago Style

Thapa, Rupak, Anil Upreti, and Bishnu Prasad Pandey. "Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal." Journal of Complementary Medicine Research 7 (2018), 66-75. doi:10.5455/jice.20171013045424

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Thapa, R., Upreti, A. & Pandey, B. P. (2018) Chemical profiling and biological activity analysis of cone, bark and needle of Pinus roxburghii collected from Nepal. Journal of Complementary Medicine Research, 7 (1), 66-75. doi:10.5455/jice.20171013045424

Contact Information

Your comments are very important to us

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access. The articles in Journal of Complementary Medicine Research are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

Copyright � 2018 Journal of Complementary Medicine Research All Rights Reserved. Subject to change without notice from or liability to Journal of Complementary Medicine Research. For best results, please use Internet Explorer or Google Chrome	Contact Information Your comments are very important to us We welcome your ideas, suggestions, comments, or questions. To reach Editorial Board please use one of the methods provided below. Call us at (302) 355 0333. Our office hours are Monday - Friday, 8 AM to 5 PM EST. Or e-mail us at info@ejmanager.com We will respond within 48 to 72 hours. Change of address or personal information? Please visit your account at www.ejmanager.com to update your information.