E-ISSN 2577-5669
 

Original Research 


Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants

Suleiman Alhaji Muhammad, Aminu Lailaba Abubakar, Sulaiman Rabiu, Ismail Sulaiman.

Abstract
Background/Aim: The medicinal plants and their derivatives have long been recognized as important sources of antioxidants in the prevention and treatment of various diseases. This study investigated phytochemicals, antioxidant content and in vitro DPPH free radical scavenging activities of methanolic and aqueous leaves extracts of Anogeissus leiocarpus, Ipomoea asarifolia, Bauhinia rufescens, Guiera. senegalensis and Moringa oleifera.
Materials and Methods: The extracts were subjected to qualitative phytochemical analysis to identify the bioactive constituents in each plant. Total phenolics and proanthocyanidin contents were determined using Folin–Ciocalteu and vanillin-methanol assays while antioxidant free scavenging activity was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.
Results: The phytochemical screening revealed the presence of alkaloids, flavonoids, and tannins in all the five plants investigated. The total phenolic and proanthocyanidin contents of methanolic extracts were significantly higher (p<0.05) as compared with aqueous extracts. DPPH free radical scavenging activity of methanolic extracts of A. leiocarpus and M. oleifera was similar to vitamin C. In vitro DPPH radical scavenging activity of methanolic extracts of A. leiocarpus and M. oleifera were better as compared with B. refescens, I. asarifolia, G. senegalensis and aqueous extracts. The antioxidant activities of both extracts were in a dose-dependent manner. Furthermore, the lower IC50 of methanolic extracts of A. leiocarpus, B. refescens, and M. oleifera correlated with strong scavenging activities of these plants.

Conclusion: This study demonstrated the antioxidant content and DPPH free radical scavenging activities of five medicinal plants. Thus, the study underscored these plants as potential sources of natural antioxidants that can be explored for the treatment of oxidant-related diseases.

Key words: Antioxidants, DPPH, free radicals, medicinal plants, phytochemicals


 
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Pubmed Style

Muhammad SA, Abubakar AL, Rabiu S, Sulaiman I. Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. J Complement Med Res. 2018; 7(1): 57-65. doi:10.5455/jice.20170926111217


Web Style

Muhammad SA, Abubakar AL, Rabiu S, Sulaiman I. Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. http://www.jocmr.com/?mno=279003 [Access: April 23, 2018]. doi:10.5455/jice.20170926111217


AMA (American Medical Association) Style

Muhammad SA, Abubakar AL, Rabiu S, Sulaiman I. Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. J Complement Med Res. 2018; 7(1): 57-65. doi:10.5455/jice.20170926111217



Vancouver/ICMJE Style

Muhammad SA, Abubakar AL, Rabiu S, Sulaiman I. Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. J Complement Med Res. (2018), [cited April 23, 2018]; 7(1): 57-65. doi:10.5455/jice.20170926111217



Harvard Style

Muhammad, S. A., Abubakar, A. L., Rabiu, S. & Sulaiman, I. (2018) Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. J Complement Med Res, 7 (1), 57-65. doi:10.5455/jice.20170926111217



Turabian Style

Muhammad, Suleiman Alhaji, Aminu Lailaba Abubakar, Sulaiman Rabiu, and Ismail Sulaiman. 2018. Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. Journal of Complementary Medicine Research, 7 (1), 57-65. doi:10.5455/jice.20170926111217



Chicago Style

Muhammad, Suleiman Alhaji, Aminu Lailaba Abubakar, Sulaiman Rabiu, and Ismail Sulaiman. "Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants." Journal of Complementary Medicine Research 7 (2018), 57-65. doi:10.5455/jice.20170926111217



MLA (The Modern Language Association) Style

Muhammad, Suleiman Alhaji, Aminu Lailaba Abubakar, Sulaiman Rabiu, and Ismail Sulaiman. "Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants." Journal of Complementary Medicine Research 7.1 (2018), 57-65. Print. doi:10.5455/jice.20170926111217



APA (American Psychological Association) Style

Muhammad, S. A., Abubakar, A. L., Rabiu, S. & Sulaiman, I. (2018) Antioxidant Content and In vitro Diphenylpicrylhydrazyl Free Radical Scavenging Activity of Selected Medicinal Plants. Journal of Complementary Medicine Research, 7 (1), 57-65. doi:10.5455/jice.20170926111217





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