E-ISSN 2577-5669
 

Original Research 


Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti.

Abstract
Aim: The main aims of the study were to evaluate the phytochemicals, antioxidant, antibacterial and chemical constituents of storage medicinal plant materials used as a traditional medicine in Nepal.
Methods: Phytochemical screening, total phenolic content, total flavonoid content, antibacterial activities, anti-oxidant assay of the crude extract (water, methanol, n-hexane and acetone) were carried out to identify the biological activities and phytonutrients present in the different extract. The chemical constituents present in the crude extract were analyzed using the high performance liquid chromatography (HPLC) equipped with UV detector.
Results: Evaluated medicinal plant materials were found to have diverse phytonutrients. Results revealed that methanol extract of Pakhanved and Jethimadhu have highest total flavonoids and polyphenol content. Among the selected medicinal plant materials Jethimadhu extract revealed the highest antioxidant activities. Furthermore, evaluated medicinal plants extract were found to exert a range of in vitro growth inhibition activity against both gram positive and gram negative species. The highest antibacterial activities were observed in the case of methanol extract, whereas, least activity was observed with the hexane extract. HPLC analysis of the acetone extract of Jethimadhu reveals the presence of diosmetin.
Conclusions: Our result revealed that among the five evaluated medicinal plant materials, Jethimadhu extract revealed biological activities and exhibits a higher amount of polyphenol and flavonoid content.

Key words: Phytochemical screening, total phenol content, total flavonoid content, antioxidant, HPLC


 
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Pubmed Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. J Complement Med Res. 2017; 6(4): 369-377. doi:10.5455/jice.20171029093516


Web Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. http://www.jocmr.com/?mno=272572 [Access: December 12, 2018]. doi:10.5455/jice.20171029093516


AMA (American Medical Association) Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. J Complement Med Res. 2017; 6(4): 369-377. doi:10.5455/jice.20171029093516



Vancouver/ICMJE Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. J Complement Med Res. (2017), [cited December 12, 2018]; 6(4): 369-377. doi:10.5455/jice.20171029093516



Harvard Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti (2017) Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. J Complement Med Res, 6 (4), 369-377. doi:10.5455/jice.20171029093516



Turabian Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. 2017. Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. Journal of Complementary Medicine Research, 6 (4), 369-377. doi:10.5455/jice.20171029093516



Chicago Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. "Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal." Journal of Complementary Medicine Research 6 (2017), 369-377. doi:10.5455/jice.20171029093516



MLA (The Modern Language Association) Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti. "Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal." Journal of Complementary Medicine Research 6.4 (2017), 369-377. Print. doi:10.5455/jice.20171029093516



APA (American Psychological Association) Style

Bishnu Prasad Pandey, Rupak Thapa, Anil Upreti (2017) Evaluation of biological activities and chemical constituent of storage medicinal plant materials used as a traditional medicine in Nepal. Journal of Complementary Medicine Research, 6 (4), 369-377. doi:10.5455/jice.20171029093516





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