E-ISSN 2577-5669
 

Original Research 


Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi.

Cited by (11)

Abstract
Background/Aim: Wounds are the outcome of injuries to the skin that interrupt the soft tissue. Healing of a wound is a complex and long-drawn-out process of tissue repair and remodeling in response to injury. A large number of plants are used by folklore traditions for treatment of cuts, wounds and burns. Moringa oleifera is an herb used as traditional folk medicine for the treatment of various skin wounds and associated diseases. The underlying mechanisms of wound healing activity of ethyl acetate fraction of M. oleifera leaves extract are completely unknown. Methods: In the current study, ethyl acetate fraction of Moringa oleifera leaves was investigated for its efficacy on cell viability, proliferation and migration (wound closure rate) in human normal dermal fibroblast cells. Results: Results revealed that lower concentration (12.5 µg/ml, 25 µg/ml, and 50 µg/ml) of ethyl acetate fraction of M. oleifera leaves showed remarkable proliferative and migratory effect on normal human dermal fibroblasts. Conclusion: The present study suggested that ethyl acetate fraction of M. oleifera leaves might be a potential therapeutic agent for skin wound healing by promoting fibroblast proliferation and migration through increasing the wound closure rate corroborating its traditional use.

Key words: Active fractions, migration, cell proliferation, scratch assay, natural products.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sivapragasam Gothai
Articles by Palanisamy Arulselvan
Articles by Woan Sean Tan
Articles by Sharida Fakurazi
on Google
on Google Scholar
Article Statistics
 Viewed: 2600
Downloaded: 702
Cited: 11

REFERENCES
1. Flanagan M. Wound measurement: Can it help us to monitor progression to healing? J Wound Care 2003;12:189-94.
2. Jalalpure SS, Agrawal N, Patil MB, Chimkode R, Tripathi A. Antimicrobial and wound healing activities of leaves of Alternanthera sessilis Linn. Int J Green Pharm 2008;2:141-4. [DOI via Crossref]   
3. Mathieu D, Linke JC, Wattel F. Non-healing wounds. Handbook on Hyperbaric Medicine. Vol. 5. Netherlands: Springer; 2006. p. 401-27.
4. Arnold M, Barbul A. Nutrition and wound healing. Plast Reconstr Surg 2006;117 7 Suppl:42S-58.
5. Vileikyte L. Stress and wound healing. Clin Dermatol 2007;25:49-55. [DOI via Crossref]    [Pubmed]   
6. Patil DN, Kulkarni AR, Shahapurkar AA, Hatappakki BC. Natural cumin seeds for wound healing activity in albino rats. Int J Biochem 2009;3:148-52. [DOI via Crossref]   
7. Whitney JD. Overview: Acute and chronic wounds. Nurs Clin North Am 2005;40:191-205, v.
8. Broughton G nd, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006;117 7 Suppl:12S-34.
9. Robson MC, Steed DL, Franz MG. Wound healing: Biologic features and approaches to maximize healing trajectories. Curr Probl Surg 2001;38:72-140. [DOI via Crossref]    [Pubmed]   
10. Caffee HH, Bingham HG. Leukopenia and silver sulfadiazine. J Trauma 1982;22:586-7. [DOI via Crossref]   
11. Thakur R, Jain N, Pathak R, Sandhu SS. Practices in wound healing studies of plants. Evid Based Complement Alternat Med 2011;2011:438056. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
12. Chitra MB, Patil R. Preliminary phytochemical investigation and wound healing activity of Allium. Cepalin (Liliaceae). Int J Pharm Pharm Sci 2009;2:7-9.
13. Mughal MH, Ali G, Srivastava PS, Iqbal M. Improvement of drumstick (Moringa pterygosperma Gaertn.) – A unique source of food and medicine through tissue culture. Hamdard Med 1999;42:37-42.
14. Farooq F, Meenu R, Avinash T, Abdul A, Sahila F. Medicinal properties of Moringa oleifera: An overview of promising healer. J Med Plants Res 2012;6:4368-74.
15. Dillard CJ, German JB. Phytochemicals: Nutraceuticals and human health. J Sci Food Agric 2000;80:1744-56. [DOI via Crossref]   
16. Estrella MP, Mantaring JV, David GZ. A double blind, randomised controlled trial on the use of malunggay (Moringa oleifera) for augmentation of the volume of breastmilk among non-nursing mothers of preterm infants. J Pediatr 2000;49:3-6.
17. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem 2003;51:2144-55. [DOI via Crossref]    [Pubmed]   
18. Atsukwei D, Eze ED, Moses DM, Adinoyi SS, Upkabi CN. Hypolipidaemic effect of ethanol leaf extract of Moringa Oleifera Lam. in experimentally induced hypercholesterolemic Wistar rats. Int J Nutr Food Sci 2014;3:355-60. [DOI via Crossref]   
19. Shestopalov AV, Shkurat TP, Mikashinovich ZI, Kryzhanovskaia IO, Bogacheva MA, Lomteva SV, et al. Biological functions of allantoin. Izv Akad Nauk Ser Biol 2006;541-5.
20. D'Souza KM, Malhotra R, Philip JL. G protein-coupled receptor kinase-2 is a novel regulator of collagen synthesis in adult human cardiac fibroblasts. J Biol Chem 2011;17:15507-16. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
21. Tan WS, Arulselvan P, Karthivashan G, Fakurazi S. Moringa oleifera flower extract suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 Macrophages via NF-?B pathway. Mediators Inflamm 2015;2015:720171.
22. Muhammad AA, Pauzi NA, Arulselvan P, Abas F, Fakurazi S. In vitro wound healing potential and identification of bioactive compounds from Moringa oleifera Lam. Biomed Res Int 2013;2013:974580. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
23. Hurd TA. Nutrition and wound-care management/prevention. Clin Pract 2004;2:20-8.
24. Menke NB, Ward KR, Witten TM, Bonchev DG, Diegelmann RF. Impaired wound healing. Clin Dermatol 2007;25:19-25. [DOI via Crossref]    [Pubmed]   
25. Zouboulis CC, Makrantonaki E. Clinical aspects and molecular diagnostics of skin aging. Clin Dermatol 2011;29:3-14. [DOI via Crossref]    [Pubmed]   
26. Chitra S, Patil MB, Ravi K. Wound healing activity of Hyptis suaveolens (L) poit (Laminiaceae). Int J Pharm Technol 2009;1:737-44.
27. Cheenpracha S, Park EJ, Yoshida WY, Barit C, Wall M, Pezzuto JM, et al. Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg Med Chem 2010;18:6598-602. [DOI via Crossref]    [Pubmed]   
28. Liang CC, Park AY, Guan JL. In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2007;2:329-33. [DOI via Crossref]    [Pubmed]   
29. Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: A Comprehensive Review. Adv Wound Care (New Rochelle) 2014;3:445-464. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
30. Hajiaghaalipour F, Kanthimathi MS, Sanusi J, Rajarajeswaran J. White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage. Food Chem 2015;169:401-10. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

LincRNA TINCR facilitates excessive proliferation and inflammation in post-burn skin fibroblasts by directly binding with SND1 protein and inducing SND1-mediated TGF-β1 expression
Biochemical and Biophysical Research Communications 2019; 509(4): 903.

1
 
A critical inquiry into marble-burying as a preclinical screening paradigm of relevance for anxiety and obsessive–compulsive disorder: Mapping the way forward
Cogn Affect Behav Neurosci 2019; 19(1): 1.

2
 
Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
BMC Complement Altern Med 2019; 19(1): .

3
 
Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation
Journal of Ethnopharmacology 2019; 234(): 27.

4
 
Moringa Genus: A Review of Phytochemistry and Pharmacology
Front. Pharmacol. 2018; 9(): .

5
 
In Vitro Wound Healing Potential of Stem Extract of Alternanthera sessilis
Evidence-Based Complementary and Alternative Medicine 2018; 2018(): 1.

6
 
The Combined Effect of Amniotic Membrane and Moringa oleifera Leaves Derived Gel for Wound and Burn Healing in Rat Model
Regen. Eng. Transl. Med. 2018; (): .

7
 
MORINGA OLEIFERA LEAF EXTRACT LOADED HYDROGEL FOR DIABETIC WOUND HEALING
MJMR 2018; 2(2): 35.

8
 
Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation
Journal of Ethnopharmacology 2018; (): .

9
 
Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
Molecules 2016; 21(12): 1452.

10
 
Role of Antioxidants and Natural Products in Inflammation
Oxidative Medicine and Cellular Longevity 2016; 2016(): 1.

11
 
How to Cite this Article
Pubmed Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. J Complement Med Res. 2016; 5(1): 1-6. doi:10.5455/jice.20160201055629


Web Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. http://www.jocmr.com/?mno=211090 [Access: April 21, 2019]. doi:10.5455/jice.20160201055629


AMA (American Medical Association) Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. J Complement Med Res. 2016; 5(1): 1-6. doi:10.5455/jice.20160201055629



Vancouver/ICMJE Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. J Complement Med Res. (2016), [cited April 21, 2019]; 5(1): 1-6. doi:10.5455/jice.20160201055629



Harvard Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi (2016) Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. J Complement Med Res, 5 (1), 1-6. doi:10.5455/jice.20160201055629



Turabian Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. 2016. Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. Journal of Complementary Medicine Research, 5 (1), 1-6. doi:10.5455/jice.20160201055629



Chicago Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. "Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts." Journal of Complementary Medicine Research 5 (2016), 1-6. doi:10.5455/jice.20160201055629



MLA (The Modern Language Association) Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi. "Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts." Journal of Complementary Medicine Research 5.1 (2016), 1-6. Print. doi:10.5455/jice.20160201055629



APA (American Psychological Association) Style

Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi (2016) Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts. Journal of Complementary Medicine Research, 5 (1), 1-6. doi:10.5455/jice.20160201055629





Most Viewed Articles
  • Role of Cyperus rotundus oil in decreasing hair growth
    GHADA FAROUK ABD EL-KAREAM
    J Intercult Ethnopharmacol. 2012; 1(2): 111-118
    » Abstract & References » doi: 10.5455/jice.20120626100304

  • Ethno-dentistry: popular medicinal plants used for dental diseases in India
    Amit Bhardwaj, Shalu Verma Bhardwaj
    J Intercult Ethnopharmacol. 2012; 1(1): 62-65
    » Abstract & References » doi: 10.5455/jice.20120322035152

  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Intercult Ethnopharmacol. 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Intercult Ethnopharmacol. 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952

  • Herbal Antibacterials: A Review
    Chirag Modi, Shailesh Mody, Hitesh Patel, Ghanshyam Dudhatra, Avinash Kumar, Madhavi Awale
    J Intercult Ethnopharmacol. 2012; 1(1): 52-61
    » Abstract & References » doi: 10.5455/jice.20120207113423

  • Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze
    Tesfaye Mohammed, Chalachew Teshale
    J Intercult Ethnopharmacol. 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jice.20120401054654

  • Medicinal plants with potential anti-arthritic activity:
    Manjusha Choudhary, Vipin Kumar, Hitesh Malhotra, Surender Singh
    J Intercult Ethnopharmacol. 2015; 4(2): 147-179
    » Abstract & References » doi: 10.5455/jice.20150313021918

  • Phytochemical and antibacterial activity of the extracts of Fagara zanthoxyloides on selected cariogenic and enteric bacterial isolates
    Martins Ajibade Adefisoye, Kwashie AjibadeAko-Nai, Mary Adejumoke Bisi-Johnson
    J Intercult Ethnopharmacol. 2012; 1(1): 1-6
    » Abstract & References » doi: 10.5455/jice.55-1333123486

  • Effect of Momordica charantia (bitter melon) on serum glucose level and various protein parameters in acetaminophen intoxicated rabbits
    Kanwal Zahra, Muhammad Imran Sohail, Muhammad Anwar Malik
    J Intercult Ethnopharmacol. 2012; 1(1): 7-12
    » Abstract & References » doi: 10.5455/jice.20120403013109

  • Study of antinociceptive and anti-inflammatory activities of certain Iranian medicinal plants
    Fariba Sharififar, Payam Khazaeli, Narguess Alli, Elham Talebian, Raheleh Zarehshahi, Shivasadat Amiri
    J Intercult Ethnopharmacol. 2012; 1(1): 19-24
    » Abstract & References » doi: 10.5455/jice.20120227104636

  • Most Downloaded
  • Medicinal plants with potential anti-arthritic activity:
    Manjusha Choudhary, Vipin Kumar, Hitesh Malhotra, Surender Singh
    J Intercult Ethnopharmacol. 2015; 4(2): 147-179
    » Abstract & References » doi: 10.5455/jice.20150313021918

  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Intercult Ethnopharmacol. 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326

  • Herbal Antibacterials: A Review
    Chirag Modi, Shailesh Mody, Hitesh Patel, Ghanshyam Dudhatra, Avinash Kumar, Madhavi Awale
    J Intercult Ethnopharmacol. 2012; 1(1): 52-61
    » Abstract & References » doi: 10.5455/jice.20120207113423

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Intercult Ethnopharmacol. 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952

  • Use of Aqueous Extract of Corn Silk in the Treatment of Urinary Tract Infection
    Ahmed Salih Sahib, Imad Hashim Mohammed, Saba Jasim Hamdan
    J Intercult Ethnopharmacol. 2012; 1(2): 93-96
    » Abstract & References » doi: 10.5455/jice.20120525123150

  • Role of Cyperus rotundus oil in decreasing hair growth
    GHADA FAROUK ABD EL-KAREAM
    J Intercult Ethnopharmacol. 2012; 1(2): 111-118
    » Abstract & References » doi: 10.5455/jice.20120626100304

  • Ethno-dentistry: popular medicinal plants used for dental diseases in India
    Amit Bhardwaj, Shalu Verma Bhardwaj
    J Intercult Ethnopharmacol. 2012; 1(1): 62-65
    » Abstract & References » doi: 10.5455/jice.20120322035152

  • A review on ethnopharmacological potential of Aloe vera L.
    Md. Shamim Hossain, A.N.M Mamun-Or-Rashid, Nayeem Md. Towfique, Monokesh Kumer Sen
    J Intercult Ethnopharmacol. 2013; 2(2): 113-120
    » Abstract & References » doi: 10.5455/jice.20130612035300

  • Phytochemical and antibacterial activity of the extracts of Fagara zanthoxyloides on selected cariogenic and enteric bacterial isolates
    Martins Ajibade Adefisoye, Kwashie AjibadeAko-Nai, Mary Adejumoke Bisi-Johnson
    J Intercult Ethnopharmacol. 2012; 1(1): 1-6
    » Abstract & References » doi: 10.5455/jice.55-1333123486

  • Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze
    Tesfaye Mohammed, Chalachew Teshale
    J Intercult Ethnopharmacol. 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jice.20120401054654

  • Most Cited Articles
  • Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium
    Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh
    J Intercult Ethnopharmacol. 2012; 1(2): 101-104
    » Abstract & References » doi: 10.5455/jice.20120525014326
    Cited : 36 times [Click to see citing articles]

  • Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro
    Emmanuel Anyachukwu Irondi, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, Francis O. Shode
    J Intercult Ethnopharmacol. 2016; 5(2): 122-130
    » Abstract & References » doi: 10.5455/jice.20160321115402
    Cited : 16 times [Click to see citing articles]

  • Potential antimalarials from African natural products: a review
    Bashir Lawal, Oluwatosin Kudirat Shittu, Adamu Yusuf Kabiru, Ali Audu Jigam, Maimuna Bello Umar, Eustace Bonghan Berinyuy, Blessing Uchenna Alozieuwa
    J Intercult Ethnopharmacol. 2015; 4(4): 318-343
    » Abstract & References » doi: 10.5455/jice.20150928102856
    Cited : 15 times [Click to see citing articles]

  • Some pharmacological effects of cinnamon and ginger herbs in obese diabetic rats
    Mostafa Abbas Shalaby, Hamed Yahya Saifan
    J Intercult Ethnopharmacol. 2014; 3(4): 144-149
    » Abstract & References » doi: 10.5455/jice.20140818050741
    Cited : 13 times [Click to see citing articles]

  • Antimicrobial and phytochemical evaluation of the leaf, stem bark and root extracts of Cyathula prostrata (L) Blume against some human pathogens
    Gideon Ikechukwu Ogu, Williams Omotola Tanimowo, Perpetua Ugonna Nwachukwu, Bright Esegbuyota Igere
    J Intercult Ethnopharmacol. 2012; 1(1): 35-43
    » Abstract & References » doi: 10.5455/jice.20120407085952
    Cited : 12 times [Click to see citing articles]

  • Herbal medicine use among Turkish patients with chronic diseases
    Münevver Tulunay, Cenk Aypak, Hülya Yıkılkan, Süleyman Görpelioğlu
    J Intercult Ethnopharmacol. 2015; 4(3): 217-220
    » Abstract & References » doi: 10.5455/jice.20150623090040
    Cited : 12 times [Click to see citing articles]

  • Medicinal plants with potential anti-arthritic activity:
    Manjusha Choudhary, Vipin Kumar, Hitesh Malhotra, Surender Singh
    J Intercult Ethnopharmacol. 2015; 4(2): 147-179
    » Abstract & References » doi: 10.5455/jice.20150313021918
    Cited : 12 times [Click to see citing articles]

  • Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts
    Sivapragasam Gothai, Palanisamy Arulselvan, Woan Sean Tan, Sharida Fakurazi
    J Intercult Ethnopharmacol. 2016; 5(1): 1-6
    » Abstract & References » doi: 10.5455/jice.20160201055629
    Cited : 11 times [Click to see citing articles]

  • In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates
    Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou
    J Intercult Ethnopharmacol. 2016; 5(3): 212-218
    » Abstract & References » doi: 10.5455/jice.20160331064446
    Cited : 11 times [Click to see citing articles]

  • Biological synthesis of silver nanoparticles from Adansonia digitata L. fruit pulp extract, characterization, and its antimicrobial properties
    Chennareddy Maruthi Kesava Kumar, Pulicherla Yugandhar, Nataru Savithramma
    J Intercult Ethnopharmacol. 2016; 5(1): 79-85
    » Abstract & References » doi: 10.5455/jice.20160124113632
    Cited : 11 times [Click to see citing articles]