E-ISSN 2577-5669
 

Original Research 


Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia

Milica Zivkovic, Neza Cadez, Ksenija Uroic, Marija Miljkovic, Maja Tolinacki, Petra Dousova, Blazenka Kos, Jagoda Suskovic, Peter Raspor, Ljubisa Topisirovic, Natasa Golic.

Cited by (6)

Abstract
Aim. The aim of this study was to investigate the in vitro probiotic potential of dairy yeast isolates from artisanal cheeses manufactured in Serbia and Croatia.
Methods. Twelve yeast strains isolated in from artisanal fresh soft and white brined cheeses manufactured in Serbia and Croatia were used in the study. Survival in chemically-simulated gastrointestinal conditions, adherence to epithelial intestinal cells and proliferation of gut-associated lymphoid tissue (GALT) cells were evaluated.
Results. The results revealed that two strains of Kluyvereomyces lactis ZIM 2408 and ZIM 2453 grew above one log unit (Δ log CFU/ml) in the complex colonic medium during 24 h of cultivation, while Torulaspora delbrueckii ZIM 2460 was the most resistant isolate in chemically-simulated conditions of gastric juice and upper intestinal tract. It was demonstrated that the strains Kluyvereomyces lactis ZIM 2408 and ZIM2441 and Saccharomyces cerevisiae ZIM 2415 were highly adhesive to Caco-2 cells, while strains Kluyvereomyces lactis ZIM 2408 and Debaryomyces hansenii ZIM 2415 exhibit the highest adhesion percentage to HT29-MTX cells. All strains significantly (p<0.0001) decreased the proliferation of gut-associated lymphoid tissue (GALT) cells suggesting the possible strain-specific immunomodulatory potential of the isolates.
Conclusion. The dairy yeast isolates exhibit the strain-specific probiotic properties. Particularly, the strain K. lactis ZIM 2408 appears to be the best probiotic candidate in terms of all three criteria. Taking into account their immunomodulatory potential, the yeast isolates could be further tested for specific probiotic applications and eventually included in functional food formulated for patients suffering from diseases associated with an increased inflammatory status.

Key words: Caco-2, HT29-MTX, GALT, host-microbe interactions, immunomodulation, Kluyvereomyces lactis


 
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How to Cite this Article
Pubmed Style

Zivkovic M, Cadez N, Uroic K, Miljkovic M, Tolinacki M, Dousova P, Kos B, Suskovic J, Raspor P, Topisirovic L, Golic N. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. J Complement Med Res. 2015; 4(1): 12-18. doi:10.5455/jice.20141128051842


Web Style

Zivkovic M, Cadez N, Uroic K, Miljkovic M, Tolinacki M, Dousova P, Kos B, Suskovic J, Raspor P, Topisirovic L, Golic N. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. http://www.jocmr.com/?mno=169612 [Access: July 17, 2018]. doi:10.5455/jice.20141128051842


AMA (American Medical Association) Style

Zivkovic M, Cadez N, Uroic K, Miljkovic M, Tolinacki M, Dousova P, Kos B, Suskovic J, Raspor P, Topisirovic L, Golic N. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. J Complement Med Res. 2015; 4(1): 12-18. doi:10.5455/jice.20141128051842



Vancouver/ICMJE Style

Zivkovic M, Cadez N, Uroic K, Miljkovic M, Tolinacki M, Dousova P, Kos B, Suskovic J, Raspor P, Topisirovic L, Golic N. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. J Complement Med Res. (2015), [cited July 17, 2018]; 4(1): 12-18. doi:10.5455/jice.20141128051842



Harvard Style

Zivkovic, M., Cadez, N., Uroic, K., Miljkovic, M., Tolinacki, M., Dousova, P., Kos, B., Suskovic, J., Raspor, P., Topisirovic, L. & Golic, N. (2015) Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. J Complement Med Res, 4 (1), 12-18. doi:10.5455/jice.20141128051842



Turabian Style

Zivkovic, Milica, Neza Cadez, Ksenija Uroic, Marija Miljkovic, Maja Tolinacki, Petra Dousova, Blazenka Kos, Jagoda Suskovic, Peter Raspor, Ljubisa Topisirovic, and Natasa Golic. 2015. Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. Journal of Complementary Medicine Research, 4 (1), 12-18. doi:10.5455/jice.20141128051842



Chicago Style

Zivkovic, Milica, Neza Cadez, Ksenija Uroic, Marija Miljkovic, Maja Tolinacki, Petra Dousova, Blazenka Kos, Jagoda Suskovic, Peter Raspor, Ljubisa Topisirovic, and Natasa Golic. "Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia." Journal of Complementary Medicine Research 4 (2015), 12-18. doi:10.5455/jice.20141128051842



MLA (The Modern Language Association) Style

Zivkovic, Milica, Neza Cadez, Ksenija Uroic, Marija Miljkovic, Maja Tolinacki, Petra Dousova, Blazenka Kos, Jagoda Suskovic, Peter Raspor, Ljubisa Topisirovic, and Natasa Golic. "Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia." Journal of Complementary Medicine Research 4.1 (2015), 12-18. Print. doi:10.5455/jice.20141128051842



APA (American Psychological Association) Style

Zivkovic, M., Cadez, N., Uroic, K., Miljkovic, M., Tolinacki, M., Dousova, P., Kos, B., Suskovic, J., Raspor, P., Topisirovic, L. & Golic, N. (2015) Evaluation of probiotic potential of yeasts isolated from traditional cheeses manufactured in Serbia and Croatia. Journal of Complementary Medicine Research, 4 (1), 12-18. doi:10.5455/jice.20141128051842





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    J Intercult Ethnopharmacol. 2012; 1(1): 19-24
    » Abstract & References » doi: 10.5455/jice.20120227104636

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  • Is there a role for homeopathy in breast cancer surgery? A first randomized clinical trial on treatment with Arnica montana to reduce postoperative seroma and bleeding in patients undergoing total mastectomy
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