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Original Research 


Preliminary phytochemical screening and evaluation of antibacterial activity of <i>Dichrocepala integrifolia </i> (L.f) O. kuntze

Tesfaye Mohammed, Chalachew Teshale.

Cited by (6)

Abstract
Background: More than 80% of all common disease in Ethiopia is of infectious origin. Traditional medicine plays a great role in combating this disease. Dichrocepala integrifolia (L.f) O.kuntze is one of the traditional remedies used for treatment of wound infection.
Objective: To screen the major phytochemical constituents and evaluate antibacterial activity tests of leaf extracts of Dichrocephala integrifolia (L.f.) O. kuntze.
Methods: experimental study involving gradient extraction, disc diffusion and broth dilution were conducted. Phytochemical screenings were also done to assess the presence of major secondary metabolites.
Result: Chloroform extracts were the most effective where as ethanol extract were the least effective. S. typhi and pseudomonas aeruginosa were found to be the most sensitive and resistant microorganisms to all extract used at all test concentration, respectively. Accordingly from all test strains, S.typhi was inhibited by the minimum concentration of chloroform extract (MIC=125mg/ml).
Conclusion and Recommendation: In general the antimicrobial activity of the plant observed here support the tradition therapeutic claim of the society. Further study should be conducted in further evaluating its antimicrobial effectiveness and also purification of the active chemical constituents that could be responsible for its biological activity.

Key words: Antibacterial activity, phytochemical screening, disc diffusion, broth dilution, <i>Dichrocepala integrifolia</i>


 
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How to Cite this Article
Pubmed Style

Mohammed T, Teshale C. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. J Complement Med Res. 2012; 1(1): 30-34. doi:10.5455/jice.20120401054654


Web Style

Mohammed T, Teshale C. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. http://www.jocmr.com/?mno=13811 [Access: September 19, 2018]. doi:10.5455/jice.20120401054654


AMA (American Medical Association) Style

Mohammed T, Teshale C. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. J Complement Med Res. 2012; 1(1): 30-34. doi:10.5455/jice.20120401054654



Vancouver/ICMJE Style

Mohammed T, Teshale C. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. J Complement Med Res. (2012), [cited September 19, 2018]; 1(1): 30-34. doi:10.5455/jice.20120401054654



Harvard Style

Mohammed, T. & Teshale, C. (2012) Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. J Complement Med Res, 1 (1), 30-34. doi:10.5455/jice.20120401054654



Turabian Style

Mohammed, Tesfaye, and Chalachew Teshale. 2012. Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. Journal of Complementary Medicine Research, 1 (1), 30-34. doi:10.5455/jice.20120401054654



Chicago Style

Mohammed, Tesfaye, and Chalachew Teshale. "Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze." Journal of Complementary Medicine Research 1 (2012), 30-34. doi:10.5455/jice.20120401054654



MLA (The Modern Language Association) Style

Mohammed, Tesfaye, and Chalachew Teshale. "Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze." Journal of Complementary Medicine Research 1.1 (2012), 30-34. Print. doi:10.5455/jice.20120401054654



APA (American Psychological Association) Style

Mohammed, T. & Teshale, C. (2012) Preliminary phytochemical screening and evaluation of antibacterial activity of Dichrocepala integrifolia (L.f) O. kuntze. Journal of Complementary Medicine Research, 1 (1), 30-34. doi:10.5455/jice.20120401054654





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