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Original Research 


Preliminary phytochemical analysis and cytotoxic potential of <i>Cucumis trigonus</i> Roxb

Balakrishnan Arunachalam, Kokilavani Chinnaraju.

Cited by (2)

Abstract
Aim: The present study involves in the investigation of various extracts of Cucumis trigonus Roxb. for its phytochemical constituents and also the cytotoxic potential of the Cucumis trigonus Roxb. ethanolic fruit extract.
Methods: The phytochemical screening with methanol, ethanol, petroleum ether, chloroform and aqueous extracts of Cucumis trigonus Roxb. was done by modern method of Peach and Tracey (1955) Cytotoxicity is estimated by staining technique using Trypan blue after the addition of drug, the dead cells are stained blue with Trypan blue. Confirmation studies were done by additional metabolic intervention experiments like (3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assays.
Results: Qualitative phytochemical analysis of the plant extracts were done and it was found that the presence of phytochemicals were maximum in the ethanolic extract when compared to other extracts. So the ethanolic extract of C. trigonus fruit was used for further investigations. In the cytotoxic study the ethanolic fruit extract concentration at 1.25 mg / ml was found to be the effective dose because at this concentration, it exhibited 50% cytotoxicity against Hep2 cells.
Conclusion: These results conclude that the ethanolic fruit extract of Cucumis trigonus possess a good phytochemical strength and also an effective cytotoxic potential.

Key words: Cucumis trigonus, phytochemical, Trypan blue, Cytotoxicity


 
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This Article Cited By the following articles

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Comp Clin Pathol 2018; 27(2): 295.

1
 
In vitro analysis of antispasmodic activity of ethanolic stem extracts of Uvaria rufa Blume and Anomianthus dulcis (Dunal) J. Sinclair on excised rat’s ileum
Comp Clin Pathol 2017; (): .

2
 
How to Cite this Article
Pubmed Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Complement Med Res. 2012; 1(1): 13-18. doi:10.5455/jice.20120117121046


Web Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. http://www.jocmr.com/?mno=13783 [Access: October 16, 2019]. doi:10.5455/jice.20120117121046


AMA (American Medical Association) Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Complement Med Res. 2012; 1(1): 13-18. doi:10.5455/jice.20120117121046



Vancouver/ICMJE Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Complement Med Res. (2012), [cited October 16, 2019]; 1(1): 13-18. doi:10.5455/jice.20120117121046



Harvard Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju (2012) Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Complement Med Res, 1 (1), 13-18. doi:10.5455/jice.20120117121046



Turabian Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. 2012. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. Journal of Complementary Medicine Research, 1 (1), 13-18. doi:10.5455/jice.20120117121046



Chicago Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. "Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb." Journal of Complementary Medicine Research 1 (2012), 13-18. doi:10.5455/jice.20120117121046



MLA (The Modern Language Association) Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju. "Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb." Journal of Complementary Medicine Research 1.1 (2012), 13-18. Print. doi:10.5455/jice.20120117121046



APA (American Psychological Association) Style

Balakrishnan Arunachalam, Kokilavani Chinnaraju (2012) Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. Journal of Complementary Medicine Research, 1 (1), 13-18. doi:10.5455/jice.20120117121046





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